BioXtal™(高通量)蛋白结晶条件筛选试剂盒

相比近年来有长足进展的生物样品纯化和晶体X射线衍射技术，生物大分子晶体的培养依然是一个试验探索的过程，极大程度地依赖于经验并带有较大的盲目性。 蛋白结晶所需的各种成

    相比近年来有长足进展的生物样品纯化和晶体X射线衍射技术，生物大分子晶体的培养依然是一个试验探索的过程，极大程度地依赖于经验并带有较大的盲目性。 蛋白结晶所需的各种成分，如沉淀剂、盐、缓冲液体系、有机溶剂以及各种添加剂，构成一个庞大的多维集合。虽然现在市场上有一些商业化的晶体生长条件筛选盒,但它们只能构成一个一个独立的小子集, 远远无法覆盖非常复杂、巨大的蛋白结晶条件集合。

    博亚的BioXtal™结晶条件筛选盒是为蛋白晶体生长条件筛选而特别开发的一个晶体生长条件组合。 其成分组成是以大量发表和未发表的结晶统计数据为基础发展而成。它既包含离散矩阵筛选模式也包括系统性筛选模式，既含公认最有效的结晶条件也含许多其它结晶条件筛选盒所未有的条件。 扩展了筛选范围从而增大了发现结晶条件的几率。

    该试剂盒有96个条件以适合96孔板自动化筛选设备，pH范围涵盖蛋白晶体最易生长范围4.5-8.5。所使用的化学试剂来自国标公认化学试剂公司（Fluka、Sigma-Aldrich等），并且每种溶液都经过0.22μ虑膜过虑灭菌处理。96个条件分别密封在螺旋盖试管中，每管不少于10毫升，可随便使用。

      在最近进行的大量蛋白晶体筛选测试研究中，博亚BioXtal™结晶条件筛选盒曾结晶出了超过50%的可结晶蛋白。被认为是高效结晶条件组合，是一个对现有商业化的结晶条件筛选盒的有力补充和发展。

BioXtalTM结晶条件筛选试剂盒

 

 1.  0.9M (NH4)2SO4 ,0.1M Na-acetate pH4.5

 2. 0.9M (NH4)2SO4 ,0.02M HEPES pH7.5 ,0.7M NaNO3

 3. 1.5M (NH4)2SO4 ,0.1M MES pH6.5

 4. 1.5M (NH4)2SO4 ,0.1M Bis-tris pH7.0,

     0.7%(w/v) Octyl-β-D-glucopyranoside

 5. 1.5M (NH4)2SO4  ,0.1M Tris-HCl pH7.5,

     0.05M MgCl2

 6. 2.0M (NH4)2SO4  ,0.1M Na-citrate pH5.0,

     0.5M NaNO3

 7. 2.0M (NH4)2SO4 ,0.1M Bis-tris pH5.5

 8. 2.0M (NH4)2SO4  ,0.1M Bis-tris pH6.5

 9. 2.0M (NH4)2SO4 ,0.02M HEPES pH7.5

10. 2.0M (NH4)2SO4 ,0.1M Tris-HCl pH8.0,

     0.02M CaCl2 ,0.5% Octyl-β-D-glucopyranoside

11. 2.0M (NH4)2SO4 ,0.1M Tris-HCl pH8.5

12. 2.0M (NH4)2SO4

13. 2.88M (NH4)2SO4 ,0.1M Na-citrate pH5.0

14. 2.88M (NH4)2SO4 ,0.02M HEPES pH7.5

15. 15.0%(v/v) Jeffamine M600 ,0.1M HEPES pH7.0

16. 30.0%(w/v) Jeffamine ED-2001 ,0.1M HEPES pH7.0

17. 1.8M Li2SO4 ,0.05M Na Cacodylate pH6.0,

     0.01M MgSO4

18. 2.0M Li2SO4 ,0.05M MES pH5.6 ,0.01M MgCl2

19. 15.0%(w/v) PEG 2000 MME ,0.1M Tris-HCl pH7.5 ,

     0.2M KCl

20. 20.0%(w/v) PEG 2000 MME ,0.1M Tris-HCl pH8.5,

    0.2M trimethylamine n-oxide

21. 28.0%(w/v) PEG 2000 MME ,0.1M Bis-tris pH6.5

22. 30.0%(w/v) PEG 2000 MME ,

     0.05M Na Cacodylate pH6.0 ,0.05M MgCl2

23. 30.0%(w/v) PEG 2000 MME ,0.15M KBr

24. 20.0%(w/v) PEG 5000 MME ,0.1M Bis-tris pH6.5

25. 20.0%(w/v) PEG 5000 MME ,0.1M HEPES pH7.0,

     0.5M NaNO3

26. 25.0%(w/v) PEG 5000 MME ,0.1M Tris-HCl pH7.5

27. 25.0%(v/v) PEG 550 MME ,0.1M Na-citrate pH5.0

28. 25.0%(v/v) PEG 550 MME ,0.1M Tris-HCl pH8.5

29. 30.0%(v/v) PEG 550 MME ,0.1M Bis-tris pH7.0 ,

     0.05M MgCl2

30. 10.0%(v/v) MPD ,0.1M Na-citrate pH5.0

31. 10.0%(v/v) MPD ,0.1M Tris-HCl pH7.5 0.1M CaCl2

32. 20.0%(v/v) MPD ,0.05M Na Cacodylate pH6.0

33. 25.0%(v/v) MPD ,0.1M Na-citrate pH5.0

34. 25.0%(v/v) MPD ,0.1M MES pH6.5 ,0.75M NaNO3

35. 25.0%(v/v) MPD ,0.02M HEPES pH7.5 ,0.05M MgCl2

36. 30.0%(v/v) MPD ,0.1M Na-acetate pH4.5

37. 30.0%(v/v) MPD ,0.1M MES 6.5 ,0.02M CaCl2

38. 30.0%(v/v) MPD ,0.1M Tris-HCl pH8.5 ,0.02M MgCl2

39. 40.0%(v/v) MPD ,0.01M CaCl2

40. 10.0%(v/v) MPD ,0.1M Tris-HCl pH7.5 ,0.01M MgCl2 ,

     0.05M NH4-acetate

41. 1.2M NaCl ,0.1M MES pH6.5

42. 1.2M NaCl ,0.6M NaNO3 ,

     0.25%(w/v) Octyl-β-D-glucopyranoside

43. 1.5M NaCl ,0.1M Na-acetate pH4.5

44. 1.5M NaCl ,0.1M Bis-tris pH7.0

45. 1.5M NaCl ,0.1M Tris-HCl pH8.5 ,0.01M CaCl2 ,

     0.4%(w/v)  Octyl-β-D-glucopyranoside

46. 1.5M NaCl ,0.1M Tris-HCl pH8.5 ,0.6M NaNO3

47. 1.5M NaCl 0.1M NH4-acetate ,

     0.4% Octyl-β-D-glucopyranoside

48. 2.0M NaCl ,0.02M HEPES 7.5

49. 2.0M NaCl ,0.3M NaNO3

50. 2.5M NaCl ,0.1M Na-citrate pH5.0 ,

     0.9M NaNO3

51. 2.5M NaCl ,0.1M Bis-tris pH6.5 ,0.3M NaNO3

52. 2.5M NaCl ,0.1M Bis-tris pH7.0 ,

     0.1M NH4-acetate

 

53. 2.5M NaCl ,0.02M HEPES pH7.5 ,0.05M MgCl2

54. 2.5M NaCl ,0.02M HEPES pH7.5

55. 2.9M NaCl ,0.1M Tris-HCl pH8.5,

     0.4%(w/v) Octyl-β-D-glucopyranoside

56. 3.0M NaCl ,0.1M Na-citrate pH5.0 ,0.5M NaNO3

57. 3.1M NaCl ,0.1M Bis-tris pH7.0

58. 3.5M NaCl ,0.1M Bis-tris pH5.5

59. 3.5M NaCl ,0.1M Tris-HCl pH7.5 ,0.05M MgCl2

60. 30.0%(w/v) PEG 1000 ,0.1M Tris-HCl pH7.5 ,

     5.0%(v/v) Glycerol

61. 17.0%(w/v) PEG 10000 ,0.1M Bis-tris pH5.5 ,

     0.1M NH4-acetate

62. 15.0%(w/v) PEG 3350 ,0.1M MES pH6.5 ,0.85M NaNO3

63. 17.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5

64. 20.0%(w/v) PEG 3350 ,0.02M HEPES pH7.5 ,

     0.05M MgCl2

65. 20.0%(w/v) PEG 3350 ,0.2M Na-formate

66. 21.0%(w/v) PEG 3350 ,0.02M HEPES pH7.5,

     0.7% Octyl-β-D-glucopyranoside

67. 22.0%(w/v) PEG 3350 ,0.1M MES pH6.5 ,0.08M CaCl2 ,

     0.7% Octyl-β-D-glucopyranoside

68. 24.0%(w/v) PEG 3350 ,0.01M CaCl2

69. 25.0%(w/v) PEG 3350 ,0.1M Na-citrate pH5.0

70. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5 ,

     0.2M Li2SO4

71. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5,

     0.2M NaCl

72. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5

73. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5,

     0.2M NH4-acetate

74. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5 ,

    0.2M MgCl2

75. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH6.5 ,

    0.2M MgCl2

76. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH6.5 ,

     0.2M NH4-acetate

77. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5 ,

     0.2M MgCl2

78. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5 ,0.2M NaCl

79. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5

80. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5 ,

     0.2M NH4-acetate

81. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5,

     0.2M (NH4)2SO4

82. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5 ,   

    0.2M NaCl

83. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5

84. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5 ,

     0.2M NH4-acetate

85. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5 ,

     0.2M MgCl2

86. 28.0%(w/v) PEG 3350 ,0.1M Bis-tris pH 7.0 ,

     0.02M CaCl2

87. 30.0%(w/v) PEG 3350 ,0.1M Na-citrate pH5.0

88. 30.0%(w/v) PEG 3350 ,0.4M NaNO3

89. 40.0%(w/v) PEG 3350 ,0.1M Na-citrate pH5.0

90. 40.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH7.5 ,

     0.4M NaNO3

91. 30.0%(v/v) PEG 400 ,0.05M Na Cacodylate pH6.0

92. 30.0%(v/v) PEG 400 ,0.1M HEPES pH7.5 ,0.2M MgCl2

93. 35.0%(v/v) PEG 400 ,0.1M Bis-tris pH6.5 ,

     0.1M NH4-acetate

94. 25.0%(w/v) PEG 4000 ,0.1M Na-acetate pH4.6 ,

     0.2M (NH4)2SO4

95. 30.0%(v/v)  pentaerythritol ethoxylate  (15/4 EO/OH) ,

     0.05M Bis-tris pH6.5  ,0.05M (NH4)2SO4

96. 35.0%(v/v) pentaerythritol propoxylate (5/4 PO/OH) ,

     0.05M HEPES pH7.5 ,0.2M KCl

 

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